Technical University of Denmark
Browse (361.55 kB)

PEG3000 titration of Ddx4 liquid-liquid phase separation using FIDA

Download (361.55 kB)
posted on 2021-04-19, 08:15 authored by Emil G. P. Stender, Rasmus Krogh NorrildRasmus Krogh Norrild
The N-terminal of human deadbox helicase 4 (Ddx4n1) is a well established model system of protein condensate formation also called liquid-liquid phase separation. This experiment explores the effect of polyethylene glycol 3000 (PEG3000) on the thermodynamics of condensate formation using flow induced capillary flow experiments (Capflex). Condensates form when the solution is cooled in the capillary and is then measured after some time. The excel file contains specifications on the data files, which is used together with the analysis python script (10.11583/DTU.14223116).

More information on the data can be found in the linked publication