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Supplementary materials for publication "Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system" in New Biotechnology

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ONT sequencing data for manuscript "A split β-lactamase complementation based in-frame selection system for fast and efficient increase of functional diversity of antibody phage display libraries"

The data is divided in barcodes 1 to 10, which correspond to:

  • Barcode 1: Mix 1 in unselective plate
  • Barcode 2: Mix 1 in selective plate
  • Barcode 3: Mix 2 in unselective plate
  • Barcode 4: Mix 2 in selective plate
  • Barcode 5: Mix 3 in unselective plate
  • Barcode 6: Mix 3 in selective plate
  • Barcode 7: Mix 4 in unselective plate
  • Barcode 8: Mix 4 in selective plate
  • Barcode 9: Library in unselective plate
  • Barcode 10: Library in selective plate

IGV can be used to analyze data in each barcode by comparing to the reference sequence "pTP_AlBla-7B6pRE-OmBla_fix.fa". The ONT sequencing report can also be found. 


This work was supported by The Novo Nordisk Foundation Grant NNF19SA0056783, NNF19SA0057794, and NNF20SA0066621


Related publications (DOI or link to DTU Orbit, DTU Findit)

ORCID for corresponding depositor