Technical University of Denmark
Browse
ONT_data.zip (754.81 MB)

Supplementary materials for publication "Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system" in New Biotechnology

Download (754.81 MB)
dataset
posted on 2023-03-07, 14:22 authored by Oscar Mejias Gomez, Lasse Ebdrup PedersenLasse Ebdrup Pedersen, Steffen GoletzSteffen Goletz, Peter Kristensen, Andreas Visbech MadsenAndreas Visbech Madsen

ONT sequencing data for manuscript "A split β-lactamase complementation based in-frame selection system for fast and efficient increase of functional diversity of antibody phage display libraries"


The data is divided in barcodes 1 to 10, which correspond to:

  • Barcode 1: Mix 1 in unselective plate
  • Barcode 2: Mix 1 in selective plate
  • Barcode 3: Mix 2 in unselective plate
  • Barcode 4: Mix 2 in selective plate
  • Barcode 5: Mix 3 in unselective plate
  • Barcode 6: Mix 3 in selective plate
  • Barcode 7: Mix 4 in unselective plate
  • Barcode 8: Mix 4 in selective plate
  • Barcode 9: Library in unselective plate
  • Barcode 10: Library in selective plate

IGV can be used to analyze data in each barcode by comparing to the reference sequence "pTP_AlBla-7B6pRE-OmBla_fix.fa". The ONT sequencing report can also be found. 

Funding

This work was supported by The Novo Nordisk Foundation Grant NNF19SA0056783, NNF19SA0057794, and NNF20SA0066621

History

ORCID for corresponding depositor

Usage metrics

    DTU Bioengineering

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC