Supplementary materials for publication "Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system" in New Biotechnology

<p>ONT sequencing data for manuscript "A split β-lactamase complementation based in-frame selection system for fast and efficient increase of functional diversity of antibody phage display libraries"</p> <p><br></p> <p>The data is divided in barcodes 1 to 10, which correspond to:</p> <ul> <li>Barcode 1: Mix 1 in unselective plate</li> <li>Barcode 2: Mix 1 in selective plate</li> <li>Barcode 3: Mix 2 in unselective plate</li> <li>Barcode 4: Mix 2 in selective plate</li> <li>Barcode 5: Mix 3 in unselective plate</li> <li>Barcode 6: Mix 3 in selective plate</li> <li>Barcode 7: Mix 4 in unselective plate</li> <li>Barcode 8: Mix 4 in selective plate</li> <li>Barcode 9: Library in unselective plate</li> <li>Barcode 10: Library in selective plate</li> </ul> <p>IGV can be used to analyze data in each barcode by comparing to the reference sequence "pTP_AlBla-7B6pRE-OmBla_fix.fa". The ONT sequencing report can also be found. </p>