Multiphoton Microscopy of Mouse Cochlea

<p dir="ltr">This dataset includes 2 3D projection videos obtained using Multiphoton Microscopy (MPM) of a decalcified mouse cochlea.</p><p dir="ltr">The cochlea has been sectioned into 2 halves before imaging. The videos represent two separate channels: a green channel and a red channel.<br><br>- Channel 2: detects the green fluorescence signal, with a wavelength band of 500 − 550 nm.</p><p dir="ltr">- Channel 3: detects the red fluorescence signal, with a wavelength band of 550 − 670 nm.</p><p dir="ltr">The original dataset consists of a combined stack of 272 2D images, which I then split into two stacks of 136 slices each, so the green and red channels were separated.</p><p dir="ltr">Background noise was removed, by subtracting the mean intensity values taken from small squares at an area with no sample in it.</p><p dir="ltr">The mean intensity for the green channel = 270.731</p><p dir="ltr">The mean intensity for the red channel = 192.183</p><p dir="ltr">Brightness and contrast were then adjusted to optimize the visualization.</p><p dir="ltr">The images were then color-coded using LUT in Fiji (ImageJ), symbolically representing the green channel stack as green and the red channel stack as red.</p><p dir="ltr">The final 3D projections were generated using Fiji's 3D Project function with a frame rate of 7 frames per second (fps) and a scale bar was added to the videos.</p>