Multiphoton Microscopy of Mouse Cochlea

This dataset includes 2 3D projection videos obtained using Multiphoton Microscopy (MPM) of a decalcified mouse cochlea.

The cochlea has been sectioned into 2 halves before imaging. The videos represent two separate channels: a green channel and a red channel.

- Channel 2: detects the green fluorescence signal, with a wavelength band of 500 − 550 nm.

- Channel 3: detects the red fluorescence signal, with a wavelength band of 550 − 670 nm.

The original dataset consists of a combined stack of 272 2D images, which I then split into two stacks of 136 slices each, so the green and red channels were separated.

Background noise was removed, by subtracting the mean intensity values taken from small squares at an area with no sample in it.

The mean intensity for the green channel = 270.731

The mean intensity for the red channel = 192.183

Brightness and contrast were then adjusted to optimize the visualization.

The images were then color-coded using LUT in Fiji (ImageJ), symbolically representing the green channel stack as green and the red channel stack as red.

The final 3D projections were generated using Fiji's 3D Project function with a frame rate of 7 frames per second (fps) and a scale bar was added to the videos.