SI videos for the paper "3D X-ray microscopy lights up fertilizer nanoparticles in plants".
Movies from the SI of "3D X-ray microscopy lights up fertilizer nanoparticles in plants". Movies made in Dragonfly 3D. Raw data available in https://doi.org/10.11583/DTU.28190723
S1_CLMS_MnO_5h - Movie S1. Confocal imaging of MnO NPs uptake in barley leaf.In red: chlorophyll autofluorescence from the chloroplasts; in green: DiI signal.
S2_025_SP8_video - Movie S2. 3D animation of 385 nm pixel size μCT of freeze-dried soybean leaf. Data measured at the BM47XU at SPRING8.
S3_028_SP8_video - Movie S3. 3D animation of 50 nm pixel size TXM nano CT of freeze-dried soybean leaf. Thylakoid containing chloroplasts visible in red, lighter tissues in blue and green. Data measured at the BM47XU at SPRING8
S4_1065_DANMAX - Movie S4. 3D animation of 550 nm pixel size μCT scan of living barley leaf from intact plant. Data measured at the DANMAX at MAX IV.
S5_051_257nm_ESRF_video - Movie S5. 3D animation of 257 nm pixel size μCT scan of untreated barley leaf. Data was measured at the ID16B at ESRF.
S6_053_ESRF_100nm_video - Movie S6. 3D animation of 100 nm pixel size CT scan of untreated barley leaf. Data was measured at the ID16B at ESRF.
S7_105_ESRF_video - Movie S7. 3D animation of 50 nm pixel size CT scan of barley leaf. Pore space has been infiltrated with water. Data was measured at the ID16B at ESRF.
S8_102_ESRF_Vascular_video - Movie S8. 3D animation of 50 nm pixel size CT scan of untreated barley leaf with focus on vascular tissue. Data measured at the ID16B at ESRF.
S9_060_ESRF_video - Movie S9. 3D animation of 100 nm pixel size CT scan of barley leaf infiltrated with MnO nanoparticle solution 7 hours prior to scanning. Data was measured at the ID16B at ESRF.
S10_035_ESRF_MnO_CaCl - Movie S10. 3D animation of 50 nm pixel size CT scan of barley leaf. Leaf infiltrated with CaCl2 followed MnO NP solution. Data was measured at the ID16B at ESRF.
S11_099__ESRF_3D_Video - 3D animation of 100 nm pixel size CT scan of Tomato leaf. Leaf dosed with ZnO@MSN NP solution dropped on the abaxial leaf surface. Data was measured at the ID16B at ESRF.
S12_060_ESRF_CaCl - 3D animation of 100 nm pixel size CT scan of Barley leaf. Leaf infiltrated with CaCl$_2$ solution prior to scanning as a control. Data was measured at the ID16B at ESRF.
Funding
Biocompatible nanofertilizers for targeted delivery and programmed release of essential mineral ions in crops
Novo Nordisk Foundation
Find out more...DANSCATT - Instrument Center for the Use of X-Ray Synchrotron and Neutron Sources and Free-Electron X-Ray Lenses
Danish Ministry of Higher Education and Science
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